Enzymes and Inhibitors
Apexbio Technology LLC Phusion high-fidelity DNA polymerase, 100U
Phusion high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, Phusion DNA polymerases produce PCR products with very high speed and accuracy. Also, Phusion DNA polymerases are tolerant of various inhibitors, allowing for robust amplification with minimal optimization. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. Phusion high-fidelity DNA polymerases have become the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes. Other sizes are also available. Please inqury us for quote.
Sigma Aldrich Fine Chemicals Biosciences Azocasein protease substrate | 102110-74-7 | MFCD00130592 | 1g
Azocasein protease substrate Purity: MW: | 102110-74-7 | MFCD00130592 | 1g
Echelon Biosciences Research Labs PKG Substrate, Glasstide, H2Btide - 5 mg, CAS#: 81187-14-6
PKG Substrate, Glasstide, H2Btide - 5mg , Arg-Lys-Arg-Ser-Arg-Ala-Glu-OH Category/Application: Enzyme Substrates This item is non returnable
Sigma Aldrich Fine Chemicals Biosciences beta-Glucuronidase from Escherichia coli Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay) | 9001-45-0 | MFCD00130629 | 5KU
beta-Glucuronidase from Escherichia coli Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay) | 9001-45-0 | MFCD00130629 | 5KU
Cayman Chemical Ac-DNLD-AMC 1mg
A fluorogenic caspase-3 substrate; upon enzymatic cleavage by caspase-3, AMC is released and its fluorescence can be used to quantify caspase-3 activity; AMC ex/em = 340-360/440-460 nm, respectively
Echelon Biosciences Research Labs PTDINS DiC4 500 ug
Phosphatidylinositol diC4 (PI diC4) is a synthetic, purified dibutanoyl PI.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol (PI or PtdIns) is biosynthesized from CDP-diacylglycerol and inositol by phosphatidylinositol synthase. PI is the building block for phosphoinositide polyphosphates through phosphorylation by PI-kinases and is a substrate for PI-PLC yielding diacylgelycerol. This item is non returnable
Echelon Biosciences Research Labs DRONC Substrate - 5 mg,
DRONC Substrate - 5mg , Ac-Thr-Gln-Thr-Asp-AFC Category/Application: Enzyme Substrates This item is non returnable
ABclonal Technology DNA Ligase I Rabbit mAb
This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.
INTACT GENOMICS INC Exonuclease III 10000 Units (100 units/µl)
Exonuclease III (Exo III) is an exodeoxyribonuclease that digests one strand of duplex DNA from a blunt end, 5′ overhang or nick. The enzyme acts in a 3′→5′ direction producing stretches of single-stranded DNA (1, 2). Exo III is not active on 3′ overhangs of 4 or more bases in length and ssDNA. The enzyme has also RNase H, 3′-DNA phosphatase, and apurinic DNA endonuclease activities. Exo III digestions proceed at a uniform rate yielding predictable and reproducible results. However, the observed rate of nucleotide excision can vary depending on the individual characteristics of the reaction mix such as reaction temperature, ionic strength, template base content, template helical structure, and enzyme-to-DNA ratios (3-5).Applications• 3′ → 5′ exonuclease• Production of unidirectional nested deletions• Production of intermediates for site-directed mutagenesis• Preparation of strand-specific probes• Preparation of single-stranded substrates for dideoxy sequencing
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous glycerol solution, >=4,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 2KU
Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous glycerol solution, >=4,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 2KU